"HIV"
ANTIBODY TESTING: ASSUMPTIONS & REALITIES
by Stephen C. Byrnes, N.D., Ph.D.
http://www.naturalhawaii.com/byrnes.htm
It is commonly propagated by the Centers for Disease Control (CDC), the National
Institutes of Health (NIH), most doctors, and the health media that the so-called
"HIV" antibody tests are close to 100% accurate. Whether the test used is ELISA,
IFA, or Western Blot, the preciseness of the test, and of its results, are little
questioned. There exist, however, scores of documented "false positives" to
the "HIV" test as many different biochemical processes and substances can cross-react
with the supposedly unique viral proteins of "HIV." In the scientific literature,
false positives to every single "HIV" protein have been documented (39). This,
of course, raises the obvious and embarrassing question for the CDC, NIH, et.
al. who assert that the tests are nearly 100% accurate: how does one really
know if the positive test result one receives is a genuine positive reaction
to the "HIV" antibody, or simply a cross-reaction to one of a plethora of other
things? Obviously, one does not; indeed, cannot. Since we natural health professionals
usually question everything held as sacrosanct by the allopathic medical establishment,
it is high time that we also question one of the fundamental tenets of modern
day medical terrorism: that a positive "HIV" test result is
(a) valid and (b)
an ominous portent of death from "AIDS." Since one of the main causes of "AIDS"
is the toxic stress that a positive "HIV" antibody test engenders (65), it behooves
us as therapists and practitioners to make any and all of our clients/patients
who have been told they're "HIV" positive aware of the inherent faultiness of
the tests. Similarly, it is our duty to inform our patients that the current
"marker" tests for health in "HIV" antibody positive people, e.g., so-called
"Viral Load" testing and CD4 counts, are equally invalid (67,68,69,70, 71).
We must make our clients aware that they are not going to die unless they continue
to believe in the current medical model of "AIDS," and follow recommended courses
of "treatment" for it. Such treatments invariably involve the use of nucleoside,
DNA chain terminating drugs, i.e., oral chemotherapeutic agents such as AZT,
ddI, ddC, 3TC, and d4T, assorted antibiotics (given as prophylaxis against various
bacterial infections) which decimate gut flora and, therefore, digestive function,
various antifungals which interfere with liver function, toxic sulfa drugs,
and the newer "protease inhibitors" which may increase the occurrence of immune
problems due to their interference with protein metabolism and various cellular
functions (72). Before giving the list, four points need to be kept in mind:
(A) Just because an item shows up on the
list, does not mean that the item will always cause a false positive reaction.
The overall test result depends on what antibodies an individual has, as well
as their biochemical individuality. The characteristics of the test kit being
used (they are not standardized) are also factors.
(B) The list does not indicate which test
(ELISA, IFA, or Western Blot) the item cross-reacted with. Some items more readily
cross-react with ELISA, some with WB, some with both.
(C) Point number two brings up a familiar
objection from "HIV" supporters: that genuine false positives on ELISA are screened
out by the "more accurate" WB test. This contention only begs the question more.
The WB shows positive by virtue of accumulating enough positive bands to add
up to the total number required by the criteria a lab uses to interpret it (39).
Obviously, then, the more a person has been exposed to foreign antigens, proteins,
and various infectious agents, the more antibodies a person will have circulating
in their blood. This means an increased likelihood of cross-reacting antibodies
and, therefore, an increased likelihood of a false positive WB.
(D) Following point number three, it should
be remembered that the various "AIDS risk groups," (gay men, hemophiliacs, drug
addicts, and some groups of Africans), but not the general population, have
a number of foreign antigens and proteins in their systems for a variety of
reasons (frequent sexual contacts, intake of pooled blood concentrates from
countless donors, etc.). This is why people in these groups tend to have a high
incidence of positive WB's, while the general population does not. Nevertheless,
even those from the general populace receive positive WB's for a variety of
reasons, known and unknown. It is hoped that this list will generate much discussion
and thought among practitioners. Since it is obvious from the scientific literature
that the HIV tests are inherently unreliable, and since an increasing number
of prominent scientists are questioning the very existence of HIV, as well as
all retroviruses (39, 66, 73), more attention should be paid to the non-contagious
risk factors (nutritional, toxicological, and psychological) which are the most
likely causes of "AIDS" and other immunosuppressive conditions which continue
to affect a growing number of people.
FACTORS KNOWN TO CAUSE FALSE POSITIVE
HIV ANTIBODY TEST RESULTS
Acute viral infections, including infections of DNA (59,48,43,53,40,13)
Administration of human immunoglobulin preparations pooled before 1985 (10)
Alcoholic hepatitis/alcoholic liver disease (32,48,40,10,13,49,43,53)
Alpha interferon therapy in hemodialysis patients (54) Anal sex- receptive (39,64)
Anti-carbohydrate antibodies (52,19,13)
Anti-collagen antibodies (31)
Anti-hepatitis A IgM (48)
Anti-Hbc IgM (48)
Anti-lymphocyte antibodies (56,31)
Anti-parietal cell antibody (48)
Anti-microsomal antibodies (34)
Anti-mitochondrial antibodies (48,13)
Anti-nuclear antibodies (48,13,53)
Anti-smooth muscle antibody (48)
Antibodies with a high affinity for polysterene used in the test kits (62,40,3)
Autoimmune diseases (44,29,10,40,49,43)
Blood transfusions, including multiple (63,36,13,49,43,41)
Candidiasis (66)
Epstein-Barr virus (37)
False positives on other tests, including syphilis (17,48,33,10,49)
Flu (36)
Flu vaccination (30,11,3,20,13,43)
Hemophilia (10,49)
Hematologic malignant disorders/lymphoma (43,53,9,48,13)
Healthy individuals (10)
Heat treated specimens (51,57,24,49,48)
Hemodialysis/renal failure (56,16,41,10,49)
Hepatitis (54)
Herpes simplex I & II (27,11)
Hepatitis B vaccination (28,21,40,43)
High levels of antibodies (40,33)
High levels of circulating immune complexes (6,33)
HLA antibodies (7,46,63,48,10,13,49,43)
Leprosy (2,25)
Lipemic serum (blood with high fat levels) (49)
Lupus (15,23)
Malaria (6,12)
Malignant neoplasms (40)
Multiple myeloma (10,43,53)
Mycobacterium avium (25)
Naturally-occurring antibodies (5,19)
Normal human ribonucleoproteins (48,13)
Organ transplantation (1,36)
Other retroviruses (8,55,14,48,13)
Passive immunization: receipt of gamma or immune globulin as prophylaxis against
infection which contains antibodies (18,26,60,4,22,42,43,13)
Pregnancy in multiparous women (58,53,13,43,36)
Primary biliary cirrhosis (43,53,13,48)
Proteins on the filter paper (13)
Q fever with associated hepatitis (61)
Recent viral infection or exposure to viral vaccines (11)
Renal failure (48,23,13)
Renal transplantation (35,9,48,13,56)
Rheumatoid arthritis (36)
Serum positive for rheumatoid factor and antinuclear antibody (14,62,53)
"Sticky" blood in Africans (38,34,40)
Stevens-Johnson syndrome (9,48,13)
T-cell leukocyte antigen antibodies (48,13)
Tetanus vaccination (40)
Tuberculosis (25)
Upper respiratory infections (11)
Visceral leishmaniasis (45)
The author gratefully acknowledges research done by Christine Johnson of Zenger's
California in compiling this data.
REFERENCES
1. Agbalika, Fershal, Garnier, et. al. 1992. False-positive antigens related
to emergence of a 25-30 kD protein detected in organ recipients. AIDS 6:959-962.
2. Andrade, Aveilleira, Marques, et. al. 1991. Leprosy as a cause of false-positive
results in serological assays for the detection of antibodies to HIV-1. Intl.
J. Leprosy, 59:125.
3. Arnold, Slade, Jones, et. al. 1994. Donor follow up of influenze vaccine-related
multiple viral enzyme immunoassay reactivity. Vox Sanguinis, 67:191.
4. Ascher, Roberts. 1993. Determination of the etiology of seroreversals in
HIV testing by antibody fingerprinting. AIDS, 6:241.
5. Barbacid, Bolgnesi, Aaronson. 1980. Humans have antibodies capable of recognizing
oncoviral glycoproteins. Proc. Nat'l Acad. Of Sci. 77:1617-1621.
6. Biggar, Melbye, Sarin, et. al. 1985. ELISA HTLV retrovirus antibody reactivity
associated with malaria and immune complexes in healthy Africans. Lancet ii:520-543.
7. Blanton, Balakrishan, Dumaswala, et. al. 1987. HLA antibodies in blood donors
with reactive screening tests for antibody to the immunodeficiency virus. Transfusion
27 (1):118.
8. Blomberg, Vincic, Jonsson, et al. 1990. Identification of regios of HIV-1
p24 reactive with sera which give "indeterminate" results in electrophoretic
immunoblots with the help of long synthetic peptides. AIDS Res. Hum. Retro.
6:1363/
9. Burkhardt, Mertens, Eggers. 1987. Comparison of two commercially available
anti-HIV ELISA's. J. Med. Vir. 23:217.
10. Bylund, Ziegner, Hooper. 1992. Review of testing for HIV. Clin. Lab. Med.12:305-333.
11. Challakere, Rapaport,. 1993. False-positiveHIV type 1 ELISA results in low-risk
subjects. West. J. Med. 159 (2): 214-215.
12. Charmot, Simon. 1990. HIV infection and malaria. Revue du Practicien. 40:2141.
13. Cordes, Ryan. 1995. Pitfalls in HIV testing. Post. Med. 98:177.
14. Dock, Lamberson, O'Brien, et al. 1988. Evaluation of atypical HIV immunoblot
reactivity in blood donors. Transfusion 28:142.
15. Esteva, Blasini, Ogly, et al. 1992. False positive results for antibody
to HIV in two men with systemic lupus erythematosus. Ann. Rheum. Dis. 51:1071-1073.
16. Fassbinder, Kuhni, Neumayer, et al. 1986. Prevalence of antibodies against
LAV/HTLV-III in patients with terminal renal insufficiency treated with hemodialysis
and following renal transplantation. Deutsche Medizinishe Wochenschrift 111:1087.
17. Fleming, Cochi, Steece, et al. 1987. AIDS in low-incidence areas. JAMA 258
(6) :785.
18. Gill, Rachlis, Anand. 1991. Five cases of erroneously diagnosed HIV infection.
Can. Med. Assoc. J. 145 (12): 1593.
19. Healey, Bolton,. 1993. Apparent HIV-1 glycoprotein reactivity in Western
blot in uninfected blood donors. AIDS 7:655-658.
20. Hisa, J.. 1993. False-positive ELISA for HIV after influenza vaccination.
JID, 167:989.
21. Isaacman, S. 1989. Positive HIV antibody test results after treatment with
hepatitis B immune globulin. JAMA, 262:209.
22. Jackson, Rubenis, Knigge, et al. 1988. Passive immunoneutralisation of HIV
in patients with advanced AIDS. Lancet, Sept. 17:647.
23. Jindal, Solomon, Burrows. 1993. False positive tests for HIV in a woman
with renal failure and lupus. NEJM, 328:1281-1282.
24. Jungkind, DiRenzo, Young. 1986. Effect of using heat-inactivated serum with
the Abbot human T-cell lymphotropic virus type III antibody test. J. Clin. Micro.
23:381.
25. Kashala, Marlink, Ilunga, et al. 1994. Infection with HIV type 1 (HIV-1)
and human T-cell lymphotropic virus viruses among leprosy patients and contacts.
J. Infect. Dis.169:296-304.
26. Lai-Goldman, McBride, Howanitz, et al. 1987. Presence of HTLV- III antibodies
in immune serum glogulin preparations. Am. J. Clin. Path. 87:635.
27. Langedijk, Vos, Doornum, et al. Identification of cross-reactive epitopes
recognized by HIV-1 false-positive sera. AIDS, 6:1547-1548.
28. Lee, Eby, Molinaro. 1992. HIV false positivity after hepatitis B vaccination.
Lancet 339:1060.
29. Leo-Amador, Ramirez-Rodriguez, Galvan, et al. Antibodies against HIV in
generalized lupus. Salud Publica de Mexico 32:15.
30. Mackenzie, Davis, Peterson, et al. 1992. Multiple false-positive serologic
tests for HIV, HTLV-1, and hepatitis C following influenze vaccination. JAMA,
268:1015-1017. 31. Mathe, G. 1992. Is the AIDS virus responsible for the disease?
Biomed. & Pharmoc.46:1-2.
32.Mendenhall, Roselle, Grossman. 1986. False-positive tests for HTLV-III antibodies
in alcoholic patients with hepatitis. NEJM, 314:921.
33. Moore, Cone, Alexander. 1986. HTLV-III seropositivity in 1971-1972 parenteral
drug abusers--a case of false positives or of viral exposure? NEJM, 314:1387-1388.
34. Mortimer, Mortimer, Parry. 1985. Which anti-HTLV-III/LAV assays for screening
and confirmatory testing? Lancet, Oct. 19, p. 873.
35. Neale, Dagger, Fong. 1985. False-positive anti-HTLV III serology. New Zealand
Med. J., 10/23.
36. Ng, V. 1991. Serological diagnosis with recombinant peptides/proteins. Clin.
Chem. , 37:1667-1668.
37. Ozanne & Fauvel. 1988. Performance and reliability of five commerical ELISA
kits in screening for anti-HIV antibody in high-risk subjects. J. Clin. Micro.
26:1496.
38. Papdopulos-Eleopulos, E. 1988. Reappraisal of AIDS. Med. Hypoth., 25:151.
39. Papdopulos-Eleopulos, Turner, Papadimitriou. 1993. Is a positive Western
blot proof of HIV infection? Bio/Technology June 11:696-707.
40. Pearlman, Ballas. 1994. False-positive HIV screening test related to rabies
vaccination. Arch. Pathol. Lab. Med. 118-805.
41. Peternan, Lang, Mikos. Hemodialysis/renal failure. JAMA, 255:2327.
42. Piszkewicz, D. 1987. HTLV-III antibodies after immune globulin. JAMA, 257:316.
43. Profitt, Yen-Liberman. 1993. Lab diagnosis of HIV infection. Inf. Dis. Clin.
North Am., 7:203.
44. Ranki, Kurki, Reipponen. 1992. Antibodies to retroviral proteins in autoimmune
connective tissue disease. Arth. & Rheum., 35:1483.
45. Ribeiro, Brites, Moreira. 1993. Serologic validation of HIV infection in
a tropical area. J. AIDS 6:319.
46. Savers, Beatty, hansen. 12986. HLA antibodies as a cause of false-positive
reactions in screening ELISA tests for antibodies to HIV. Transfusion, 26 (1):
114.
47. Sayre, Dodd, Tegtmeier. 1996. False-positive HIV type 1 Western blot tests
in non-infected blood donors. Transfusion, 36:45.
48. Schleupner, CJ. Detection of HIV-1 infection. Principles and Practices of
Infectious Diseases, 3rd. Ed., New York: Churchill Livingstone, 1990:1092.
49. Schoetman, George. 1992. Serologic tests for the detection of HIV infection.
In AIDS Testing Methodology and Management Issues, Springer-Verlag, New York.
50. Simonsen, Bffington, Shapiro. 1995. Multiple false reactions in viral antibody
screening assays after influenza vaccination. Am. J. Epidem., 141-1089.
51. Smith, Dewhurst, Sheperd. 1987. False-positive ELISA reactions for antibody
to HIV in a population of midwestern patients with congenital bleeding disorders.
Transfusion, 127:112.
52. Snyder, & Fleissner. 1980. Specificity of human antibodies to oncovirus
glycoproteins. Proc. Nat'l. Acad. Sci. 77:1622-1626.
53. Steckelberg & Cockerill. 1988. Serologic testing for HIV antibodies. Mayo
Clinic Proc. 63:373.
54. Sungar, Akpolat, Ozkuymcu. Alpha Interferon therapy in hemodialysis patients.
Nephron. 67:251.
55. Tribe, reed, Lindell. 1988. Antibodies reactive with HIV gag-coated antigens
are a amjor cause of ELISAreactivity in blood donor population. J. Clin. Micro.
April:641.
56. Ujhelvi, Fust, Illei. 1989. Different types of false positive anti-HIV reactions
in patients on hemodialysis. Immun. Let. 22:35-40.
57. Van Beers, Duys, Maes. Heat inactivation of serum may interfere with tests
for LAV/HTLV-III. J. Vir. Meth. 12:329.
58. Voevodin, A. 1992. HIV screening in Russia. Lancet. 339:1548.
59. Weber, Moshtaghi, Brunner. 1995. Evaluation of the reliability of six current
anti-HIV1/HIV-2 enzyme immunoassays. J. Vir. Meth. 55:97.
60. Wood, Williams, McNamara. 1986. Antibody against HIV in commercial intravenous
gammaglobulin preparations. Ann. Int. Med. 105:536.
61. Yale, Degroen, Tooson. 1994. Unusual aspects of acute Q fever-associated
hepatitis. Mayo Clinic Proc. 69:769.
62. Yoshida, matsui, Kobayashi. 1987. Evaluation of passive particle agglutination
test for antibody to HIV. J. Clin. Micro. Aug.:1433.
63. Yu & Landry. 1989. A false positive HIV antibody reaction due to transfusion-induced
HLA-DR4 sensitization. NEJM 320:1495.
64. National Institutes of Justice, AIDS Bulletin, Oct. 1988.
65. Byrnes, Stephen. Overcoming AIDS with Natural Medicine. Centuar Books: 1997;
Ellner, M. The Hypnosis of AIDS, lecture given 4/24/97 at HEAL San Francisco.
66.Eleopulos, Turner, Papadimitriou, Stewart, Causer. 1997. HIV antibodies:
further questions and a plea for clarification. Curr. Med. Res. And Opin., 13:627-634.
67. Duesberg & Bialy, Reply to Ho and Wei. Genetica, June 1995.
68. Eleopulos, Turner, Papadimitriou. Is HIV really hiding in the lymphnodes?
Reappr. AIDS, April, 1994.
69. Craddock, Mark. HIV: Science by Press Conference in AIDS: Virus or Drug
Induced? Kluwert Academic Publications: Netherlands, 1995.
70. Wolthers, KC. et. al. Telomere Length in HIV-1 Infection. Science, 724:5292:1543-1547.
71. Patik, et. al. 1993. Science, 259:1749-1753.
72. Lanka, Kremer, Hassig. 1997. AIDS: Death by Prescription. Continuum, 4:2.
73. Lanka, Stefan. 1995. HIV: Reality or Artifact? Transcript of lecture presented
by Dr. lanka at the 1995 International AIDS Symposium in Buenos Aires, Argentina.
For further information, please consult The Group for Scientific Reappraisal
of the HIV/AIDS Hypothesis at www.virusmyth.com, www.sumeria.com, or any of
the works by Peter Duesberg, Ph.D. or Stephen Byrnes, N.D., Ph.D.